Journal: Endocrinology

Article Title: Localization of glucokinase-like immunoreactivity in the rat lower brain stem: for possible location of brain glucose-sensing mechanisms.

doi: 10.1210/endo.141.1.7234

Figure Lengend Snippet: FIG. 3. Glucose transporter-like immunoreactivities in GK-like immunopositive ependymocytes and endothelial cells. The GLUT2-like im- munoreactivities were detected on the cilia of GK-like immunopositive ependymocytes composing the wall of the central canal (A), the aqueduct (B), the third ventricle (C), the lateral ventricle (D), and the fourth ventricle (E). A three-dimensional image of whole mount preparation constructed from the confocal laser scanning microscopic sections revealed that GLUT2-like immunoreactivities (white) were located along the wall of the fourth ventricle, but not on the dorsal surface of area postrema (F). Precise localization of the GLUT2 (black) on the cilia was revealed by the immunoperoxidase histochemistry (G). GLUT2-like immunoreactivity was eliminated by preincubation of anti-GLUT2 antibody with

Article Snippet: We used a rabbit polyclonal antibody to GLUT1 (1:100; Santa Cruz Biotechnology, Inc.) and GLUT4 (1:25; Chemicon International, Inc., Temecula, CA).

Techniques: Construct

Journal: Endocrinology

Article Title: Localization of glucokinase-like immunoreactivity in the rat lower brain stem: for possible location of brain glucose-sensing mechanisms.

doi: 10.1210/endo.141.1.7234

Figure Lengend Snippet: FIG. 6. The schematic representation of the distribution of GK-like immuno- reactive cells in the brain. In addition to the schema, GK-like immunopositive ependymocytes showed the GLUT2-like and GLUT1-like immunoreactivities on the cilia and the GLUT4-like immuno- reactivity predominantly in cytoplas- mic area (see Fig. 3, A–E). Endothelial cells also showed the GLUT1-like im- munoreactivity lining the wall of mi- crovessel (see Fig. 3J). 3V, Third ven- tricle; 4V, fourth ventricle; AP, area postrema; AQ, aqueduct; CC, central canal; DRn, dorsal raphe nucleus; Hip, hippocampus; LV, lateral ventricle; MRn, median raphe nucleus; RMg, ra- phe magnus; RO, raphe obscurus; RP, raphe pallidus; RPo, raphe pontis; Sep, septum.

Article Snippet: We used a rabbit polyclonal antibody to GLUT1 (1:100; Santa Cruz Biotechnology, Inc.) and GLUT4 (1:25; Chemicon International, Inc., Temecula, CA).

Techniques:

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of peritoneal macrophages on the levels of adiponectin ( ACDC ), GLUT1 , and GLUT4 transcripts in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques:

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of cytokines on the regulation of the ACDC, GLUT1, and GLUT4 genes in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Cell Culture

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Effects of recombinant TNF-α, IL-6, and IL-1β on GLUT4 protein expression. Fully differentiated adipocytes were incubated in medium containing recombinant mouse TNF-α (2 ng/ml), IL-6 (10 ng/ml), or IL-1β (20 ng/ml) for 2 days. Cells were fixed and immunostained for GLUT1 or GLUT4 expression using flow cytometry as described in Materials and Methods. The data are presented as means ± standard errors of the means for 3 to 6 independent replicates per treatment. Different letters indicate a significant difference. A P-value of <0.05 was considered significant.

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Recombinant, Expressing, Incubation, Flow Cytometry